Name: Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody
Brand: Alomone Labs
SKU: APR-020-GP

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Overview

Cat #: APR-020-GP

Alternative Name P2Y purinoceptor 12, P2RY12, ADP-glucose receptor, Platelet ADP receptor, HORK3

Lyophilized Powder yes

Type: Polyclonal

Host: Guinea pig

Reactivity: h, m, r

Immunogen

Peptide CTAENTLFYVKES, corresponding to amino acid residues 270-282 of human P2RY12 (Accession Q9H244). 3^rdextracellular loop.

Accession (Uniprot) Number Q9H244

Gene ID 64805

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse – 12/13 amino acid residues identical; rat – 12/13 amino acid residues identical.

RRID AB_2756620.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Guinea pig total IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ic, if, ih, lci, wb

May also work in: ifc*, ip*

Western blot

Rat and mouse brain lysates, rat basophilic leukemia (RBL) and human brain glioblastoma (U-87 MG) cell line lysates (1:400-1:2000).
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
1,2. Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020-GP), (1:400).
3,4. Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody, preincubated with P2Y12 Receptor (extracellular) Blocking Peptide (#BLP-PR020).
Western blot analysis of rat basophilic leukemia (RBL) cell line lysate (lanes 5 and 7) and human brain glioblastoma (U-87 MG) cell line lysate (lanes 6 and 8):
5,6. Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020-GP), (1:400).
7,8. Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody, preincubated with P2Y12 Receptor (extracellular) Blocking Peptide (#BLP-PR020).

Live cell imaging / Immunocytochemistry

Human glioblastoma (U-87 MG) cells (1:100).

Scientific background

The P2Y receptors belong to the G-protein coupled receptor superfamily. They mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDPA). Eight functional mammalian P2Y receptors have been described: P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14^1-3.

The P2Y12 receptor is co-expressed with P2Y1 receptor on platelets leading to shape change, aggregation, and rise in intracellular Ca²⁺ upon activation.

The only other expression of P2Y12 was found in brain, according to results of reverse transcription-polymerase chain reaction and northern blotting⁴. The P2Y12 receptor has become a target for potential therapeutic drugs for the treatment of thromboembolism and other clotting disorders⁴.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 20/11/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

P2Y12 Receptor (extracellular) Blocking Peptide (#BLP-PR020) is the original antigen used for immunization during Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020) generation. The blocking peptide binds and ‘blocks’ Anti-P2Y12 Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

P2Y12 Receptor (extracellular) Blocking Peptide (#BLP-PR020)

Shipping and Ordering Information

Free International Shipping

Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

For more country-specific shipping and contact information see Ordering & Shipping.

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Certificate of Analysis

Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody