Overview
Cat #:
ANR-003-GP
Alternative Name Synaptotagmin I, SytI, p65, SVP65
Lyophilized Powder yes
Type: Polyclonal
Host: Guinea Pig
Reactivity: m, r
May also work in: h*
ImmunogenPeptide MVSASHPEALAAPVTTVAT(C), corresponding to amino acid residues 1-19 of rat Synaptotagmin 1 (Accession
P21107). Intravesicular, N- terminus.
Accession (Uniprot) Number P21707
Gene ID 25716
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – 18 out of 19 amino acid residues identical, human, bovine – 17 out of 19 amino acid residues identical, chicken – 15 out of 19 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
Isotype Guinea pig total IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Reconstitution 0.2 ml double distilled water (DDW).
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ip*
Scientific background Synaptotagmin 1 is a member of the Ca2+-binding synaptotagmin family of proteins that includes 16 members in vertebrates and is involved in regulated exocytosis and trafficking1,2.
Synaptotagmins are type I membrane proteins located in intracellular vesicles and consist of a short N-terminal domain located in the vesicle lumen, a transmembrane domain and a cytosolic domain containing two Ca2+ binding domains1,2.
Synaptotagmins are usually localized to distinct secretory vesicles and control their Ca2+-dependent fusion. Synaptotagmin I is predominantly expressed in neuronal cells and is an abundant constituent of synaptic vesicles1,2.
Synaptotagmin 1 has a relatively low Ca2+ affinity and is able to bind Ca2+ mostly in the vicinity of Ca2+ channels pores. Indeed, Synaptotagmin 1 has been demonstrated to directly interact with selected Ca2+ channels. Synaptotagmin 1 also binds to the t-SNARE protein Syntaxin 1 in a Ca2+-dependent manner thus forming the prototypical Ca2+-dependent fast synaptic vesicle exocytosis protein complex that also includes SNAP-25 and VAMP1,2,3.
Lyophilized Powder
For research purposes only, not for human use
Last Update: 29/10/2024
