Name: Guinea pig Anti-TRPV4 Antibody
Brand: Alomone Labs
SKU: ACC-034-GP (formerly AGP-147)

Overview

Cat #: ACC-034-GP (formerly AGP-147)

Alternative Name OTRPC4, VRL-2, VR-OAC, TRP12

Lyophilized Powder yes

Type: Polyclonal

Host: Guinea pig

Reactivity: r

May also work in: h*, m*

Immunogen

Peptide CDGHQQGYAPKWRAEDAPL, corresponding to amino acid residues 853-871 of rat TRPV4 (Accession Q9ERZ8). Intracellular, C-terminus.

Accession (Uniprot) Number Q9ERZ8

Gene ID 66026

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - 17/19 amino acid residues identical; human - 16/19 amino acid residues identical.

RRID AB_2876796

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Guinea pig total IgG

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8mg/ml

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: wb

May also work in: ic*, ifc*, ih*, ip*

Western blot

Western blot analysis of rat brain membranes:
1. Guinea pig Anti-TRPV4 Antibody (#Acc-034-GP), (1:1500).
2. Guinea pig Anti-TRPV4 Antibody, preincubated with TRPV4 Blocking Peptide (#BLP-CC034).
Western blot analysis of rat DRG lysates:
3. Guinea pig Anti-TRPV4 Antibody (#Acc-034-GP), (1:200).
4. Guinea pig Anti-TRPV4 Antibody, preincubated with TRPV4 Blocking Peptide (#BLP-CC034).

References

Montell, C. et al. (2002) Mol. Cell. 9, 229.
Clapham, D.E. (2003) Nature 426, 517.
Moran, M.M. et al. (2004) Curr.Opin.Neurobiol. 14, 362.
Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591.
Gunthorpe, M.J. et al. (2002) Trends. Pharmacol. Sci. 23, 183.
Peng, J.B. et al. (2003) J. Physiol. 551.3, 729.

Scientific background

TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells¹. According to IUPHAR, the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP)^1-4. The TRPV subfamily consists of six members, TRPV1-6⁵.

TRPV4 (also named OTRPC4) is activated under hypotonic conditions and serves as an osmoreceptor. TRPV4 is expressed in brain, liver, kidney, heart, testis and salivary gland⁶.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Guinea pig Anti-TRPV4 Antibody (#Acc-034-GP), raised in guinea pig, is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize TRPV4 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-TRPV4 Antibody (#ACC-034) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

TRPV4 Blocking Peptide (#BLP-CC034) is the original antigen used for immunization during Anti-TRPV4 Antibody (#ACC-034) generation. The blocking peptide binds and ‘blocks’ Anti-TRPV4 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

TRPV4 Blocking Peptide (#BLP-CC034)

Shipping and Ordering Information

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Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

For more country-specific shipping and contact information see Ordering & Shipping.

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Guinea pig Anti-TRPV4 Antibody