Overview
Alomone Labs is pleased to offer the HCN Antibody Explorer Kit (#AK-205). The Explorer Kit contains HCN antibodies with their respective peptide control antigen. An ideal tool for screening purposes.
Compounds
| Product Name | Cat # | Size |
|---|---|---|
Anti-HCN1 Antibody |
APC-056 | 1 x 50 µl |
HCN1 Blocking Peptide |
BLP-PC056 | 1 x 40 µg |
Guinea pig Anti-HCN1 Antibody |
APC-056-GP | 1 x 50 µl |
HCN1 Blocking Peptide |
BLP-PC056 | 1 x 40 µg |
Anti-HCN2 Antibody |
APC-030 | 1 x 50 µl |
HCN2 Blocking Peptide |
BLP-PC030 | 1 x 40 µg |
Anti-HCN3 Antibody |
APC-057 | 1 x 50 µl |
HCN3 Blocking Peptide |
BLP-PC057 | 1 x 40 µg |
Anti-HCN3 (extracellular) Antibody |
APC-083 | 1 x 50 µl |
HCN3 (extracellular) Blocking Peptide |
BLP-PC083 | 1 x 40 µg |
Anti-HCN4 Antibody |
APC-052 | 1 x 50 µl |
HCN4 Blocking Peptide |
BLP-PC052 | 1 x 0.15 mg |
Guinea pig Anti-HCN4 Antibody |
APC-052-GP | 1 x 50 µl |
HCN4 Blocking Peptide |
BLP-PC052 | 1 x 0.15 mg |
Scientific Background
Hyperpolarization-activated cation currents (Ih) appear in the heart and the brain and have a crucial role in controlling electrical pacemaker activity, contributing to biological processes such as heartbeat, sleep-wake cycle and synaptic plasticity1,2.
The Ih currents are generated by the Hyperpolarization-activated cyclic nucleotide-gated channel family (HCN), which is comprised of four homologous members, HCN1-4.
Each HCN subunit consists of six transmembrane domains (TM), a pore region between TM5-TM6 and a binding domain for cyclic nucleotides (CNBD) in the cytoplasmic C-terminus2. The HCN subunits can form functional homomers and can also co-assemble into functionally heteromers2.
The channels are closely related to each other and share a homology of about 60%. However, their similarity decreases in the cytoplasmic N- and C-termini. The channels HCN1-4 mainly differ from each other in their speed of activation and the extent to which they are modulated by cAMP. HCN1, weakly affected by cAMP, is the fastest channel, followed by HCN2, HCN3 and HCN4.
- Hughes, D.I. et al. (2012) J. Physiol. 590.16, 3927.
- Klueva, J. et al. (2012) Neurosignals 20, 35.
