Cat #: AK-205_KIT
Size: 14 Vials
Form: Lyophilized
Anti-HCN1 Antibody
Cat #: APC-056
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)KPNSASNSRDDGNSVYPSK, corresponding to amino acid residues 6-24 of rat HCN1 (Accession Q9JKB0). Intracellular, N-terminus.
Homology
Mouse - 18/19 amino acid residues identical; human - 16/19 amino acid residues identical.
HCN1 Blocking Peptide
Cat #: BLP-PC056
Guinea pig Anti-HCN1 Antibody
Cat #: APC-056-GP
Size:
50 µl
Host: Guinea pig
Type: Polyclonal
Application:
IF, IH, WB
Reactivity:
R
Immunogen
Peptide (C)KPNSASNSRDDGNSVYPSK, corresponding to amino acid residues 6-24 of rat HCN1 (Accession Q9JKB0). Intracellular, N-terminus.
Homology
Mouse - 18/19 amino acid residues identical; human - 16/19 amino acid residues identical.
HCN1 Blocking Peptide
Cat #: BLP-PC056
Anti-HCN2 Antibody
Cat #: APC-030
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)EEAGPAGEPRGSQAS, corresponding to amino acid residues 147-161 of human HCN2 (Accession Q9UL51). Intracellular, N-terminus.
Homology
Rat, mouse - identical.
HCN2 Blocking Peptide
Cat #: BLP-PC030
Anti-HCN3 Antibody
Cat #: APC-057
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)QRATGDGSPRRKGSGSER corresponding to amino acid residues 727-744 of rat HCN3 (Accession Q9JKA8). Intracellular, C-terminus.
Homology
Human, mouse - identical.
HCN3 Blocking Peptide
Cat #: BLP-PC057
Anti-HCN3 (extracellular) Antibody
Cat #: APC-083
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, LCI, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)ELEPRLDAEVYK, corresponding to amino acid residues 190-201 of rat HCN3 (Accession Q9JKA8). 2nd extracellular loop.
Homology
Mouse, human – identical.
HCN3 (extracellular) Blocking Peptide
Cat #: BLP-PC083
Anti-HCN4 Antibody
Cat #: APC-052
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
GST fusion protein with the sequence HGHLHDSAEERRLIAEGDASPG EDRTPPGLAAEPERP, corresponding to amino acid residues 119-155 of human HCN4 (Accession Q9Y3Q4). Intracellular, N-terminus.
Homology
Rabbit - identical; rat - 35/37 amino acid residues identical.
HCN4 Blocking Peptide
Cat #: BLP-PC052
Guinea pig Anti-HCN4 Antibody
Cat #: APC-052-GP
Size:
50 µl
Host: Guinea pig
Type: Polyclonal
Application:
IF, IH, WB
Reactivity:
H, M, R
Immunogen
GST fusion protein with the sequence HGHLHDSAEE RRLIAEGDASPGEDRTPPGLAAEPERP, corresponding to amino acid residues 119-155 of human HCN4 (Accession Q9Y3Q4). Intracellular, N-terminus.
Homology
Rabbit - identical; rat - 35/37 amino acid residues identical; mouse - 33/37 amino acid residues identical.
HCN4 Blocking Peptide
Cat #: BLP-PC052
Antibody Storage & Reconstitution Instructions
Storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Add deionized water, depending on the sample size.
Storage after reconstitution
The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control antigen storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution
100 μl water.
Control antigen storage after reconstitution
-20ºC.
Preadsorption control
1 μg peptide per 1 μg antibody.
Note
Guinea pig polyclonal antibodies (#APC-056-GP & #APC-052-GP) are included in this Explorer Kit. Please take into account when reacting with a secondary antibody.
For research purposes only, not for human use
