Overview
Type: Synthetic peptide
Form: Lyophilized powder
HCN1 Blocking Peptide (#BLP-PC056) is the original antigen used for immunization during Anti-HCN1 Antibody (#APC-056) generation. The blocking peptide binds and ‘blocks’ Anti-HCN1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membrane:1. Anti-HCN1 Antibody (#APC-056), (1:200).
2. Anti-HCN1 Antibody, preincubated with HCN1 Blocking Peptide (#BLP-PC056).
Expression of HCN1 in mouse cerebellumImmunohistochemical staining mouse cerebellum using Anti-HCN1 Antibody (#APC-056). A. HCN1 (green) appears in the cerebellar pinceau. B. Calcium binding calbindinD28-K (red), a marker of Purkinje neurons, is stained in the same section. C. Merge of the images demonstrates the position of the HCN1-positive pinceau structures at the axon initial segment of Purkinje neurons.
Western blot analysis of rat brain lysate:1. Guinea pig Anti-HCN1 Antibody (#APC-056-GP), (1:500).
2. Guinea pig Anti-HCN1 Antibody, preincubated with HCN1 Blocking Peptide (#BLP-PC056).
Expression of HCN1 in rat cerebellumImmunohistochemical staining of rat cerebellum using Guinea pig Anti-HCN1 Antibody (#APC-056-GP). A. HCN1 staining (red) appears in the pinceau structures (arrows). B. DAPI is used as the counterstain (blue). C. Merged image of A and B.
Properties
Sequence
- (C)KPNSASNSRDDGNSVYPSK, corresponding to amino acid residues 6-24 of rat HCN1 (Accession Q9JKB0).
Accession (Uniprot) Number Q9JKB0
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
