Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- (C)ELEPRLDAEVYK, corresponding to amino acid residues 190-201 of rat HCN3 (Accession Q9JKA8).
Accession (Uniprot) Number Q9JKA8
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-HCN3 (extracellular) Antibody (#APC-083), (1:200).
3,4. Anti-HCN3 (extracellular) Antibody, preincubated with HCN3 (extracellular) Blocking Peptide (#BLP-PC083).
Note: Samples were heated to 37°C prior to loading on gel.
Last update: 02/01/2024
