Size: 0.15 mg
Type: GST fusion protein
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- GST fusion protein with the sequence HGHLHDSAEERRLIAEGDASPG EDRTPPGLAAEPERP, corresponding to amino acid residues 119-155 of human HCN4 (Accession Q9Y3Q4).
Accession (Uniprot) Number Q9Y3Q4
Peptide Confirmation Confirmed by DNA sequence and SDSPAGE.
Purity >95% (SDS-PAGE)
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 μl PBS.
Concentration After Reconstitution 1.2 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 3 μg fusion protein per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-HCN4 Antibody (#APC-052), (1:200).
2. Anti-HCN4 Antibody, preincubated with HCN4 Blocking Peptide (#BLP-PC052).- Mouse brain sections.
Western blot analysis of rat brain lysate:1. Guinea pig Anti-HCN4 Antibody (#APC-052-GP), (1:200).
2. Guinea pig Anti-HCN4 Antibody, preincubated with HCN4 Blocking Peptide (#BLP-PC052).- Mouse thalamus. Following a broad screen of secondary antibodies, the following was used for this application: #106-485-006 (Jackson ImmunoResearch).
Last update: 02/01/2024
