Overview
Alternative Name K+ channel toxin α-KTx 6.15
Lyophilized Powder yes
Origin Synthetic peptide
MW: 3898 Da
Purity: >98% (HPLC)
Effective concentration 1 nM - 1 μM.
Sequence IKCTLSKDCYSPCKKETGCPRAKCINRNCKCYGCS.
Modifications Disulfide bonds between Cys3-Cys24, Cys9-Cys29, Cys13-Cys19 and Cys31-Cys34.
Molecular formula C159H259N49O49S8.
Activity Hemitoxin reversibly inhibits rat voltage-gated K+ channels KV1.1 (KCNA1), KV1.2 (KCNA2) and KV1.3 (KCNA3)1.
References-Activity
- Srairi-Abid, N. et al. (2008) FEBS J. 275, 4641.
Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage of solutions Up to one week at 4°C or three months at -20°C.
Our bioassay
Alomone Labs Hemitoxin inhibits KV1.2 channel currents heterologously expressed in Xenopus oocytes.Currents were elicited by application of a 100 msec voltage ramp from a holding potential of -80 mV to 60 mV, delivered every 10 seconds. A. Time course of channel activity (current amplitude at -20 mV), before and during application of 500 and 100 nM Hemitoxin (#STH-500) as indicated by the bars. B. Example of superimposed current traces before and during application of 500 and 100 nM Hemitoxin (taken from the experiment in A).
Scientific background Hemitoxin is a 35 amino acid peptidyl toxin isolated from the Hemiscorpius lepturus scorpion venom and belongs to the αKTx6 family1. It reversibly blocks KV1.1, KV1.2 and KV1.3 voltage-gated K+ channels with an IC50 of 13 nM, 16 nM and 2 nM, respectively1. It shares 80% sequence similarity with Maurotoxin, and has a unique pairing of its four disulfide bridges1.
Target KV1.1, KV1.2, KV1.3 K+ channels
Peptide Content: 100%
Lyophilized Powder
For research purposes only, not for human use
Last Update: 07/05/2024
Specifications
Citations
Citations
