Overview
Type: Synthetic peptide
Form: Lyophilized powder
Histamine H1 Receptor/HRH1 Blocking Peptide (#BLP-HR001) is the original antigen used for immunization during Anti-Histamine H1 Receptor (HRH1) Antibody (#AHR-001) generation. The blocking peptide binds and ‘blocks’ Anti-Histamine H1 Receptor/HRH1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat heart membranes (lanes 1 and 3) and rat basophilic leukemia (RBL) cell lysates (lanes 2 and 4):1,2. Anti-Histamine H1 Receptor (HRH1) Antibody (#AHR-001), (1:200).
3,4. Anti-Histamine H1 Receptor (HRH1) Antibody, preincubated with Histamine H1 Receptor/HRH1 Blocking Peptide (#BLP-HR001).
Western blot analysis of mouse brain membranes:1. Anti-Histamine H1 Receptor (HRH1) Antibody (#AHR-001), (1:400).
2. Anti-Histamine H1 Receptor (HRH1) Antibody, preincubated with Histamine H1 Receptor/HRH1 Blocking Peptide (#BLP-HR001).
Expression of Histamine H1 receptor in mouse brainImmunohistochemical staining of mouse ventromedial hypothalamus (VMH) using Anti-Histamine H1 Receptor (HRH1) Antibody (#AHR-001). A. H1R (green fluorescence) appears in the outline of the VMH nucleus (arrows). B. DAPI (blue) counterstain labels all cell nuclei including VMH.
Properties
Sequence
- (C)HSRQYVSGLHLNRE, corresponding to amino acids 396-409 of rat HRH1 (Accession P31390).
Accession (Uniprot) Number P31390
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
