Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- RNGTRGGNDTFKC, corresponding to amino acids 161-173 of rat HRH2 (Accession P25102).
Accession (Uniprot) Number P25102
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat basophilic leukemia cells (RBL) (lanes 1 and 3) and rat brain lysates (lanes 2 and 4):1,2. Anti-Histamine H2 Receptor (HRH2) (extracellular) Antibody (#AHR-002), (1:200).
3,4. Anti-Histamine H2 Receptor (HRH2) (extracellular) Antibody, preincubated with Histamine H2 Receptor/HRH2 (extracellular) Blocking Peptide (#BLP-HR002).- Rat stomach sections (paraffin) (1:100) and mouse brain sections (frozen).
Last update: 02/01/2024
