Overview
Type: Synthetic peptide
Form: Lyophilized powder
Histamine H3 Receptor/HRH3 Blocking Peptide (#BLP-HR003) is the original antigen used for immunization during Anti-Histamine H3 Receptor (HRH3) Antibody (#AHR-003) generation. The blocking peptide binds and ‘blocks’ Anti-Histamine H3 Receptor/HRH3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2. Anti-Histamine H3 Receptor (HRH3) Antibody (#AHR-003), (1:200).
3,4. Anti-Histamine H3 Receptor (HRH3) Antibody, preincubated with Histamine H3 Receptor/HRH3 Blocking Peptide (#BLP-HR003).
Expression of Histamine H3 receptor in rat cerebellumImmunohistochemical staining of rat brain frozen sections with Anti-Histamine H3 Receptor (HRH3) Antibody (#AHR-003), (1:100), (green). A. H3R is particularly expressed in dendrites of Purkinje cells (arrows). B. Staining with mouse anti-parvalbumin (red) detected Purkinje cells and interneurons in the molecular layer. C. Merge of the two images demonstrates that the staining was restricted to dendrites of Purkinje cells. Cell nuclei were labeled with DAPI (blue) as the counterstain.
Properties
Sequence
- (C)RTRLRLDGGREAGPE, corresponding to amino acids 228-242 of rat HRH3 (Accession Q9QYN8).
Accession (Uniprot) Number Q9QYN8
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
