Overview
Type: Synthetic peptide
Form: Lyophilized powder
Homer3 Blocking Peptide (#BLP-PZ028) is the original antigen used for immunization during Anti-Homer3 Antibody (#APZ-028) generation. The blocking peptide binds and ‘blocks’ Anti-Homer3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):1,2. Anti-Homer3 Antibody (#APZ-028), (1:200).
3,4. Anti-Homer3 Antibody, preincubated with Homer3 Blocking Peptide (#BLP-PZ028).
Western blot analysis of human Jurkat T-cell leukemia cell line (lanes 1 and 3) and human MCF-7 breast adenocarcinoma cell line (lanes 2 and 4) lysates:1,2. Anti-Homer3 Antibody (#APZ-028), (1:200).
3,4. Anti-Homer3 Antibody, preincubated with Homer3 Blocking Peptide (#BLP-PZ028).
Expression of Homer3 in rat nucleus basalisImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Homer3 Antibody (#APZ-028), (1:1000), followed by goat anti-rabbit-AlexaFluor-488. Homer3 immunoreactivity (green) appears in cells of the horizontal diagonal band (HDB), (arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)RSQSADAPGPTERER, corresponding to amino acid residues 155 - 169 of rat Homer3 (Accession Q9Z2X5).
Accession (Uniprot) Number Q9Z2X5
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
