Overview
Type: Synthetic peptide
Form: Lyophilized powder
Human Adenosine A2A Receptor (extracellular) Blocking Peptide (#BLP-AR007) is the original antigen used for immunization during Anti-Human Adenosine A2A Receptor (extracellular) Antibody (#AAR-007) generation. The blocking peptide binds and ‘blocks’ Anti-Human Adenosine A2A Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human HL-60 promyelocytic leukemia cell line lysate (lanes 1 and 3) and human Jurkat T-cell leukemia cell line lysate (lanes 2 and 4):1,2. Anti-Human Adenosine A2A Receptor (extracellular) Antibody (#AAR-007), (1:200).
3,4. Anti-Human Adenosine A2A Receptor (extracellular) Antibody, preincubated with Human Adenosine A2A Receptor (extracellular) Blocking Peptide (#BLP-AR007).
Properties
Sequence
- CGQPKEGKNHSQG, corresponding to amino acid residues 146 - 158 of human adenosine A2A receptor (Accession P29274).
Accession (Uniprot) Number P29274
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
