Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- (C)RSYSTTYEERNITGTR, corresponding to amino acid residues 202 - 217 of human ASCT2 (Accession Q15758).
Accession (Uniprot) Number Q15758
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human MCF-7 breast adenocarcinoma (lanes 1 and 5), human Colo-205 colorectal adenocarcinoma (lanes 2 and 6), human PANC-1 pancreas ductal adenocarcinoma (lanes 3 and 7) and human Malme-3M melanoma cell line lysate (lanes 4 and 8):1-4. Anti-Human ASCT2/SLC1A5 (extracellular) Antibody (#ANT-083), (1:200)
5-8. Anti-Human ASCT2/SLC1A5 (extracellular) Antibody, preincubated with Human ASCT2/SLC1A5 (extracellular) Blocking Peptide (#BLP-NT083) (#BLP-NT083).
Last update: 02/01/2024
