Overview
Type: Synthetic peptide
Form: Lyophilized powder
Human BLT1 (extracellular) Blocking Peptide (#BLP-LR001) is the original antigen used for immunization during Anti-Human BLT1 (extracellular) Antibody (#ALR-001) generation. The blocking peptide binds and ‘blocks’ Anti-Human BLT1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of acute T-cell leukemia Jurkat (lanes 1 and 4), promyelocytic leukemia HL-60 (lanes 2 and 5) and acute monocytic leukemia THP-1 (lanes 3 and 6) human cell lysates:1-3. Anti-Human BLT1 (extracellular) Antibody (#ALR-001), (1:200).
4-6. Anti-Human BLT1 (extracellular) Antibody, preincubated with Human BLT1 (extracellular) Blocking Peptide (#BLP-LR001).
Properties
Sequence
- CFPRYPSEGHRAFH, corresponding to amino acid residues 168-181 of human LTB4R (Accession Q15722).
Accession (Uniprot) Number Q15722
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
