Overview
Type: Synthetic peptide
Form: Lyophilized powder
Human C5aR1 (extracellular) Blocking Peptide (#BLP-AR032) is the original antigen used for immunization during Anti-Human C5aR1 (extracellular) Antibody (#AAR-032) generation. The blocking peptide binds and ‘blocks’ Anti-Human C5aR1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of Human K562 myelogenous leukemia (lanes 1 and 4), MEG-01 chronic myelogenous leukemia cell (lanes 2 and 5), and THP-1 acute monocytic leukemia (lanes 3 and 6) cell lysates:1-3. Anti-Human C5aR1 (extracellular) Antibody (#AAR-032), (1:200).
4-6. Anti-Human C5aR1 (extracellular) Antibody, preincubated with Human C5aR1 (extracellular) Blocking Peptide (#BLP-AR032).
Properties
Sequence
- (C)DKDTLDLNTPVDK, corresponding to amino acid residues 16-28 of human C5a anaphylatoxin receptor (Accession P21730).
Accession (Uniprot) Number P21730
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
