Cat #: BLP-AR033
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- (C)DSVSYEYGDYSDLSD, corresponding to amino acid residues 4-18 of human C5a anaphylatoxin chemotactic receptor 2 (Accession Q9P296).
Accession (Uniprot) Number Q9P296
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 2 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of human MEG-O1 chronic myelogenous leukemia cell lines lysate (lanes 1 and 3) and human K562 erythroleukemia cell line lysate (lanes 2 and 4):1,2. Anti-Human C5aR2 (extracellular) Antibody (#AAR-033), (1:400).
3,4. Anti-Human C5aR2 (extracellular) Antibody, preincubated with Human C5aR2 (extracellular) Blocking Peptide (#BLP-AR033).
Last update: 02/01/2024