Overview
- Pennica, D. et al. (1995) Proc. Natl. Acad. Sci U.S.A. 92, 1142.
- Cheng, J.G. et al. (1997) J. Neurochem. 69, 2278.
- Sheng, Z. et al. (1996) Development 122, 419.
Alomone Labs Recombinant human Cardiotrophin-1 protein induces ERK1/2 MAPK phosphorylation.3T3-L1 cells were grown to 70% confluence, serum starved for 3 h and then stimulated with various concentrations of Recombinant human Cardiotrophin-1 protein (#C-200) for 10 min. The cell proteins were resolved by SDS PAGE and probed with anti-Phospho-ERK 1/2 MAPK.
Cardiotrophin-1 is a member of the neuropoietic cytokine family (LIF, CNTF, OSM IL-11, IL-6). Cardiotrophin-1 is a strong inducer of cardiac hypertrophy, exerts cardio-protective effects and plays a role in neuronal growth and differentiation.1,2
In neonatal rat, Cardiotrophin-1 has been shown to promote cardiac myocyte survival and to enhance embryonic myocyte proliferation during cardiac chamber development.3 Cardiotrophin-1 increases angiotensinogen gene transcription and induces VEGF expression in cardiac myocytes.4,5 It also induces vessel growth during cardiac remodelling, an effect that may contribute to its cardio-protective properties.
In humans, a recent report showed an increase in circulating Cardiotrophin-1 in heart failure.6 A strong correlation between the increased Cardiotrophin-1 and the severity of left ventricular systolic dysfunction was found.7
Cardiotrophin-1 is a very potent neurotrophic factor for motoneurons in long-term culture and protects neonatal rat motoneurons from axotomy-induced cell death.2,8 It was able to modulate the synthesis of specific neurotransmitters by cultured neuronal cells,9 and to promote the survival of rat dopaminergic and chick ciliary neurons.10 Recently, the potential for neuroprotection by Cardiotrophin-1 was investigated in animal models characterized by a prominent axonal degeneration.11-13
