Overview
Type: Synthetic peptide
Form: Lyophilized powder
Human Cav1.2/CACNA1C Blocking Peptide (#BLP-CC022) is the original antigen used for immunization during Anti-Human CaV1.2 (CACNA1C) Antibody (#ACC-022) generation. The blocking peptide binds and ‘blocks’ Anti-Human Cav1.2/CACNA1C primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat heart membranes:1. Anti-Human-CaV1.2 (CACNA1C) Antibody (#ACC-022), (1:200).
2. Anti-Human-CaV1.2 (CACNA1C) Antibody, preincubated with Human Cav1.2/CACNA1C Blocking Peptide (#BLP-CC022).
Properties
Sequence
- VNENTRMYIPEENHQ(C), corresponding to amino acid residues 2-15 of human CaV1.2 (exon 1B) (Accession Q13936).
Accession (Uniprot) Number Q13936
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
