Overview
Type: Synthetic peptide
Form: Lyophilized powder
Human CCR5 (extracellular) Blocking Peptide (#BLP-CR025) is the original antigen used for immunization during Anti-Human CCR5 (extracellular) Antibody (#ACR-025) generation. The blocking peptide binds and ‘blocks’ Anti-Human CCR5 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human HL-60 promyelocytic leukemia (lanes 1 and 4), human THP-1 acute monocytic leukemia (lanes 2 and 5) and human MEG-O1 Chronic myelogenous leukemia (lanes 3 and 6) cell lysates:1-3. Anti-Human CCR5 (extracellular) Antibody (#ACR-025), (1:400).
4-6. Anti-Human CCR5 (extracellular) Antibody, preincubated with Human CCR5 (extracellular) Blocking Peptide (#BLP-CR025).
Properties
Sequence
- CSSHFPYSQYQFWKN, corresponding to amino acid residues 178-192 of human CCR5 (Accession P51681).
Accession (Uniprot) Number P51681
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
