Overview
Type: Synthetic peptide
Form: Lyophilized powder
Human FPR1 (extracellular) Blocking Peptide (#BLP-FR001) is the original antigen used for immunization during Anti-Human FPR1 (extracellular) Antibody (#AFR-001) generation. The blocking peptide binds and ‘blocks’ Anti-Human FPR1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human HL-60 promyelocytic leukemia (lanes 1 and 5), human THP-1 acute monocytic leukemia (lanes 2 and 6), human T-84 colorectal carcinoma (lanes 3 and 7) and human U-87 MG glioblastoma (lanes 4 and 8) cell lysates:1-4. Anti-Human FPR1 (extracellular) Antibody (#AFR-001), (1:200).
5-8. Anti-Human FPR1 (extracellular) Antibody, preincubated with Human FPR1 (extracellular) Blocking Peptide (#BLP-FR001).
Properties
Sequence
- (C)NFSPWTNDPKERIN, corresponding to amino acid residues 179-192 of human FPR1 (Accession P21462).
Accession (Uniprot) Number P21462
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
