Overview
Type: Synthetic peptide
Form: Lyophilized powder
Human FPR2/ALX (extracellular) Blocking Peptide (#BLP-FR002) is the original antigen used for immunization during Anti-Human FPR2/ALX (extracellular) Antibody (#AFR-002) generation. The blocking peptide binds and ‘blocks’ Anti-Human FPR2/ALX (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of Human HL-60 promyelocytic leukemia (lanes 1 and 3) and K562 chronic myelogenous leukemia (lanes 2 and 4) cell lysates:1,2. Anti-Human FPR2/ALX (extracellular) Antibody (#AFR-002), (1:200).
3,4. Anti-Human FPR2/ALX (extracellular) Antibody, preincubated with Human FPR2/ALX (extracellular) Blocking Peptide (#BLP-FR002).- Human minor salivary glands (1:200) (Nelson, J.W. et al. (2014) Am. J. Physiol. 306, C178.).
Properties
Sequence
- (C)GGTPEERLKVAIT, corresponding to amino acid residues 184-196 of human FPR2/ALX (Accession P25090).
Accession (Uniprot) Number P25090
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
