Overview
Type: Synthetic peptide
Form: Lyophilized powder
Human GLUT3 (extracellular) Blocking Peptide (#BLP-GT043) is the original antigen used for immunization during Anti-Human GLUT3 (extracellular) Antibody (#AGT-043) generation. The blocking peptide binds and ‘blocks’ Anti-Human GLUT3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human U-87 MG glioblastoma cell line lysate (lanes 1 and 6), human THP-1 monocytic leukemia cell line lysate (lanes 2 and 7), human SH-SY5Y neuroblastoma cell line lysate (lanes 3 and 8), human MEG-01 megakaryoblastic leukemia cell line lysate (lanes 4 and 9) and human Jurkat T-cell leukemia cell line lysate (lanes 5 and 10):1-5. Anti-Human GLUT3 (extracellular) Antibody (#AGT-043), (1:500).
6-10. Anti-Human GLUT3 (extracellular) Antibody, preincubated with Human GLUT3 (extracellular) Blocking Peptide (#BLP-GT043).
Properties
Sequence
- (C)KEFINKTLTDKGN, corresponding to amino acid residues 39 - 51 of human GLUT3 (Accession P11169).
Accession (Uniprot) Number P11169
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
