Overview
Type: Synthetic peptide
Form: Lyophilized powder
Human GPR55 (extracellular) Blocking Peptide (#BLP-CR061) is the original antigen used for immunization during Anti-Human GPR55 (extracellular) Antibody (#ACR-061) generation. The blocking peptide binds and ‘blocks’ Anti-Human GPR55 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human NK-92MI natural killer cell (lanes 1 and 3) (1:500) and human acute monocytic leukemia THP-1 cell (lanes 2 and 4) (1:200) lysates:1, 2. Anti-Human GPR55 (extracellular) Antibody (#ACR-061).
3, 4. Anti-Human GPR55 (extracellular) Antibody, preincubated with Human GPR55 (extracellular) Blocking Peptide (#BLP-CR061) (#BLP-CR061).
Western blot analysis of human ovary adenocarcinoma OVCAR-3 cell (lanes 5 and 7) and human pancreatic carcinoma PANC-1 cell (lanes 6 and 8) lysates:5, 6. Anti-Human GPR55 (extracellular) Antibody (#ACR-061), (1:1000).
7, 8. Anti-Human GPR55 (extracellular) Antibody, preincubated with Human GPR55 (extracellular) Blocking Peptide (#BLP-CR061) (#BLP-CR061).
Western blot analysis of human HMC3 microglia cell (lanes 9 and 11) (1:500) and human chronic myelogenous leukemia MEG-O1 cell (lanes 10 and 12) (1:200) lysates:9, 10. Anti-Human GPR55 (extracellular) Antibody, (#ACR-061).
11, 12. Anti-Human GPR55 (extracellular) Antibody, preincubated with Human GPR55 (extracellular) Blocking Peptide (#BLP-CR061) (#BLP-CR061).
Properties
Sequence
- Peptide (C)HGKVEKYMSFHNMSD, corresponding to amino acid residues 160-174 of human GPR55 (Accession Q9Y2T6).
Accession (Uniprot) Number Q9Y2T6
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
