Overview
Type: Synthetic peptide
Form: Lyophilized powder
Human Histamine H4 Receptor/HRH4 (extracellular) Blocking Peptide (#BLP-HR004) is the original antigen used for immunization during Anti-Human Histamine H4 Receptor (HRH4) (extracellular) Antibody (#AHR-004) generation. The blocking peptide binds and ‘blocks’ Anti-Human Histamine H4 Receptor/HRH4 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human chronic myelogenous leukemia (K562) (lanes 1 and 3) and human promyelocytic leukemia (HL-60) (lanes 2 and 4) cell lysates:1,2. Anti-Human Histamine H4 Receptor (HRH4) (extracellular) Antibody (#AHR-004), (1:400).
3,4. Anti-Human Histamine H4 Receptor (HRH4) (extracellular) Antibody, preincubated with Human Histamine H4 Receptor/HRH4 (extracellular) Blocking Peptide (#BLP-HR004).
Properties
Sequence
- HTLFEWDFGKEIC, corresponding to amino acids 75-87 of human HRH4 (Accession Q9H3N8).
Accession (Uniprot) Number Q9H3N8
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
