Overview
Type: Synthetic peptide
Form: Lyophilized Powder
Human MRGPRX2 (extracellular) Blocking Peptide (#BLP-MR072) is the original antigen used for immunization during Anti-Human MRGPRX2 (extracellular) Antibody (#AMR-072) generation. The blocking peptide binds and ‘blocks’ Anti-Human MRGPRX2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human KU812 basophilic leukemia cell line lysate:1. Anti-Human MRGPRX2 (extracellular) Antibody (#AMR-072), (1:200).
2. Anti-Human MRGPRX2 (extracellular) Antibody, preincubated with Human MRGPRX2 (extracellular) Blocking Peptide (BLP-MR072).
Properties
Sequence
- Peptide (C)TPAWGTESTTVNGND, corresponding to amino acid residues 5 - 19 of human MRGPRX2 (Accession Q96LB1).
Accession (Uniprot) Number Q96LB1
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
