Overview
Type: Synthetic peptide
Form: Lyophilized powder
Human PAR1/F2R (extracellular) Blocking Peptide (#BLP-PR031) is the original antigen used for immunization during Anti-Human PAR1 (F2R) (extracellular) Antibody (#APR-031) generation. The blocking peptide binds and ‘blocks’ Anti-Human PAR1/F2R (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human promyelocytic leukemia HL-60 (lanes 1 and 4), human T-cell leukemia Jurkat (lanes 2 and 5), and chronic myelogenous leukemia K562 (lanes 3 and 6) cell line lysates:1-3. Anti-Human PAR1 (F2R) (extracellular) Antibody (#APR-031), (1:200).
4-6. Anti-Human PAR1 (F2R) (extracellular) Antibody, preincubated with Human PAR1/F2R (extracellular) Blocking Peptide (#BLP-PR031).
Western blot analysis of human colon cancer HT-29 (lanes 1 and 3) and human Colo-205 colon cancer (lanes 2 and 4) cell line lysates:1,2. Anti-Human PAR1 (F2R) (extracellular) Antibody (#APR-031), (1:200).
3,4. Anti-Human PAR1 (F2R) (extracellular) Antibody, preincubated with Human PAR1/F2R (extracellular) Blocking Peptide (#BLP-PR031).
Expression of PAR-1 in normal human breast and human breast carcinomaImmunohistochemical staining of paraffin-embedded human breast sections using Anti-Human PAR1 (F2R) (extracellular) Antibody (#APR-031), (1:100). PAR-1 staining is highly specific for epithelium-derived cells. A. In the normal resting breast, epithelial cells of the mammary ducts are visible using Histofine (pink). B. The breast carcinoma contains epithelium-derived malignant cells stained with DAB (brown). Hematoxilin is used as the counterstain.
Properties
Sequence
- (C)KNESGLTEYRLVSINK, corresponding to amino acid residues 61-76 of human PAR-1 (Accession P25116).
Accession (Uniprot) Number P25116
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
