Overview
Type: Synthetic peptide
Form: Lyophilized powder
Human PAR2/F2RL1 (extracellular) Blocking Peptide (#BLP-PR035) is the original antigen used for immunization during Anti-Human PAR2/F2RL1 (extracellular) Antibody (#APR-035) generation. The blocking peptide binds and ‘blocks’ Anti-Human PAR2/F2RL1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human HT-29 colon adenocarcinoma cell line lysate (lanes 1 and 6), human PANC-1 pancreatic carcinoma cell line lysate (lanes 2 and 7), human THP-1 monocytic leukemia cell line lysate (lanes 3 and 8), human Jurkat T-cell leukemia cell line lysate (lanes 4 and 9) and human MCF-7 breast adenocarcinoma cell line lysate (lanes 5 and 10):1-5. Anti-Human PAR2/F2RL1 (extracellular) Antibody (#APR-035), (1:200).
6-10. Anti-Human PAR2/F2RL1 (extracellular) Antibody, preincubated with Human PAR2/F2RL1 (extracellular) Blocking Peptide (#BLP-PR035) (#BLP-PR035).
Properties
Sequence
- (C)GKVDGTSHVTGKG, corresponding to amino acid residues 40 - 52 of human PAR2/F2RL1 (Accession P55085).
Accession (Uniprot) Number P55085
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Standard Quality Control Of Each Lot Western blot analysis.
