Name: Human proBDNF Basic Research Pack
Brand: Alomone Labs
SKU: ESB-601_PACK

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Cat #: ESB-601_PACK

Size: 6 Vials

Form: Lyophilized

Antibodies

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-proBDNF Antibody Cat #: ANT-006

Size: 0.2 ml Host: Rabbit Type: Polyclonal Application: IF, IH, IP, WB Reactivity: H, M, R

Immunogen

Peptide (C)DEDQKVRPNEENNKDAD, corresponding to amino acid residues 72-88 of human BDNF (precursor) (Accession P23560). Pro-domain of the BDNF protein.

Homology

Mouse, rat - 16/17 amino acid residues identical.

Anti-p75 NGF Receptor (extracellular) Antibody Cat #: ANT-007

Size: 0.2 ml Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, LCI, WB Reactivity: H, M, R

Immunogen

Peptide CEEIPGRWITRSTPPE, corresponding to amino acid residues 188-203 of human p75^NTR (Accession P08138). Extracellular, stalk domain.

Homology

Mouse - identical; rat - 15/16 amino acid residues identical.

Pharmacological Reagents

Recombinant human proBDNF protein Cat #: B-257

Size: 25 µg MW: 51.78 kDa Purity: >98% (HPLC) Net peptide content: 100%

Origin

Recombinant, E. coli

Effective concentration

0.1-10 nM

Activity

BDNF is a neurotrophic factor and binds p75^NTR as well as TrkB receptors^1,2. BDNF supports the survival of many cell types^3-8. proBDNF has been shown to be a pro-apoptotic ligand for sympathetic neurons⁹ expressing both p75 and sortilin, and to be involved in LTD¹⁰.

Storage before reconstitution

The product is shipped as a lyophilized powder at room temperature. Upon receipt, the lyophilizate should be stored at -20°C. Protect from light and moisture.

Reconstitution

Sterile water at a concentration of at least 10 µg/ml. Centrifuge all product preparations before use (10000 x g 5 min). Repeated freezing/thawing might result in loss of activity.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Recombinant human proBDNF (cleavage resistant) protein Cat #: B-256

Size: 5 µg MW: 51.45 kDa Purity: >98% (HPLC) Net peptide content: 100%

Origin

Recombinant, E. coli

Effective concentration

0.1-10 nM.

Activity

Storage before reconstitution

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Sterile water at a concentration of at least 10 µg/ml. Centrifuge all product preparations before use (10000 x g 5 min). Repeated freezing/thawing might result in loss of activity.

Storage after reconstitution

Up to one week at 4°C or four-six weeks at -70°C.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For more information, please refer to individual certificate of analysis for each product.

For research purposes only, not for human use

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Order Summary

Certificate of Analysis

Human proBDNF Basic Research Pack