Cat #: ESD-601_PACK
Size: 13 Vials
Form: Lyophilized
Antibodies
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Species reactivity key:
H- Human, M- Mouse, R- Rat
Anti-proBDNF Antibody Cat #: ANT-006
Size:
0.2 ml
Host: Rabbit
Type: Polyclonal
Application:
IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)DEDQKVRPNEENNKDAD, corresponding to amino acid residues 72-88 of human BDNF (precursor) (Accession P23560). Pro-domain of the BDNF protein.
Homology
Mouse, rat - 16/17 amino acid residues identical.
Anti-proBDNF-ATTO Fluor-488 Antibody Cat #: ANT-006-AG
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
FC, IF, IH
Reactivity:
H, M, R
Immunogen
Peptide (C)DEDQKVRPNEENNKDAD, corresponding to amino acid residues 72-88 of human BDNF (precursor) (Accession P23560). Prodomain of the BDNF protein.
Homology
Mouse, rat - 16 /17 amino acid residues identical.
Label
ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 – 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC.
Guinea pig Anti-proBDNF Antibody Cat #: ANT-006-GP
Size:
0.2 ml
Host: Guinea pig
Type: Polyclonal
Application:
IF, IH, WB
Reactivity:
H, M
Immunogen
Peptide (C)DEDQKVRPNEENNKDAD, corresponding to amino acid residues 72-88 of human BDNF (precursor) (Accession P23560). Pro-domain of the BDNF protein.
Homology
Mouse, rat - 16/17 amino acid residues identical.
Anti-p75 NGF Receptor (extracellular) Antibody Cat #: ANT-007
Size:
0.2 ml
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IFC, IH, IP, LCI, WB
Reactivity:
H, M, R
Immunogen
Peptide CEEIPGRWITRSTPPE, corresponding to amino acid residues 188-203 of human p75NTR (Accession P08138). Extracellular, stalk domain.
Homology
Mouse - identical; rat - 15/16 amino acid residues identical.
Anti-p75 NGF Receptor (extracellular)-ATTO Fluor-550 Antibody Cat #: ANT-007-AO
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, LCI
Reactivity:
H, M, R
Immunogen
Peptide CEEIPGRWITRSTPPE corresponding to amino acid residues 188-203 of human p75NTR (Accession P08138). Extracellular, stalk domain.
Homology
Mouse - identical; rat - 15/16 amino acid residues identical.
Label
ATTO-550. Maximum absorption 554 nm; maximum fluorescence 576 nm. The fluorescence is excited most efficiently in the 540 - 565 nm range. This label is related to the dye Rhodamine 6G and can be used with filters used to detect Rhodamine.
Pharmacological Reagents
Recombinant human proBDNF protein Cat #: B-257
Size:
25 µg
MW: 51.78 kDa
Purity: >98% (HPLC)
Net peptide content: 100%
Origin
Recombinant, E. coli
Effective concentration
0.1-10 nM
Activity
BDNF is a neurotrophic factor and binds p75NTR as well as TrkB receptors1,2. BDNF supports the survival of many cell types3-8. proBDNF has been shown to be a pro-apoptotic ligand for sympathetic neurons9 expressing both p75 and sortilin, and to be involved in LTD10.
Storage before reconstitution
The product is shipped as a lyophilized powder at room temperature. Upon receipt, the lyophilizate should be stored at -20°C. Protect from light and moisture.
Reconstitution
Sterile water at a concentration of at least 10 µg/ml. Centrifuge all product preparations before use (10000 x g 5 min). Repeated freezing/thawing might result in loss of activity.
Storage after reconstitution
The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.
Recombinant human proBDNF (cleavage resistant) protein Cat #: B-256
Size:
5 µg
MW: 51.45 kDa
Purity: >98% (HPLC)
Net peptide content: 100%
Origin
Recombinant, E. coli
Effective concentration
0.1-10 nM.
Activity
BDNF is a neurotrophic factor and binds p75NTR as well as TrkB receptors1,2. BDNF supports the survival of many cell types3-8. proBDNF has been shown to be a pro-apoptotic ligand for sympathetic neurons9 expressing both p75 and sortilin, and to be involved in LTD10.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Sterile water at a concentration of at least 10 µg/ml. Centrifuge all product preparations before use (10000 x g 5 min). Repeated freezing/thawing might result in loss of activity.
Storage after reconstitution
Up to one week at 4°C or four-six weeks at -70°C.
human proBDNF-Biotin Cat #: B-256-B
Size:
10 µg
MW: 51 kDa
Purity: >98%
Net peptide content: 100%
Origin
Recombinant, E. coli
Effective concentration
0.1-10 nM.
Activity
BDNF is a neurotrophic factor and binds p75NTR as well as TrkB receptors1,2. BDNF supports the survival of many cell types3-8. proBDNF has been shown to be a pro-apoptotic ligand for sympathetic neurons9 expressing both p75 and sortilin, and to be involved in LTD10.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
The product is lyophilized in a conical vial containing maximum volume of 0.5 ml. Reconstitute in any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.
Antibody Storage & Reconstitution Instructions
Storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Add deionized water, depending on the sample size.
Storage after reconstitution
The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control antigen storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution
100 μl water.
Control antigen storage after reconstitution
-20ºC.
Preadsorption control
1 μg peptide per 1 μg antibody.
For more information, please refer to individual certificate of analysis for each product.
Note
A guinea pig polyclonal antibody (#ANT-006-GP) is included in this Research Pack. Please take into account when reacting with a secondary antibody.
For research purposes only, not for human use
