Name: Human proNGF Basic Research Pack
Brand: Alomone Labs
SKU: ESB-652_PACK

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Cat #: ESB-652_PACK

Size: 6 Vials

Form: Lyophilized

Antibodies

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-proNGF Antibody Cat #: ANT-005

Size: 0.2 ml Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: H, M, R

Immunogen

Peptide (C)SPRVLFSTQPPPTSSDTLDLD, corresponding to amino acid residues 84-104 of rat NGF (precursor) (Accession P25427). Pro-domain of the NGF protein.

Homology

Mouse - identical; human - 16/21 amino acid residues identical.

Anti-p75 NGF Receptor (extracellular) Antibody Cat #: ANT-007

Size: 0.2 ml Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, LCI, WB Reactivity: H, M, R

Immunogen

Peptide CEEIPGRWITRSTPPE, corresponding to amino acid residues 188-203 of human p75^NTR (Accession P08138). Extracellular, stalk domain.

Homology

Mouse - identical; rat - 15/16 amino acid residues identical.

Pharmacological Reagents

Recombinant human proNGF protein Cat #: N-280

Size: 25 µg MW: 50.2 kDa Purity: >98% (HPLC) Net peptide content: 100%

Origin

Recombinant, E. coli

Effective concentration

0.4-4 nM.

Activity

proNGF is the preferred ligand of p75^NTR ¹. In contrast to its mature form proNGF induces apoptosis via p75^NTR ¹, but can also mediate cell survival in DRGs².

Storage before reconstitution

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Sterile water, DMEM at a concentration of at least 10 µg/ml. Centrifuge all product preparations before use (10000 x g 5 min). Repeated freezing/thawing might result in loss of activity.

Storage after reconstitution

Up to one week at 4°C or four-six weeks at -70°C.

Recombinant human proNGF (cleavage resistant) protein Cat #: N-285

Size: 10 µg MW: 50 kDa Purity: >98% (HPLC) Net peptide content: 100%

Origin

Recombinant, E. coli

Effective concentration

0.4 - 4 nM.

Activity

proNGF is the preferred ligand of p75^{NTR 1}. In contrast to its mature form proNGF induces apoptosis via p75^{NTR 1}, but can also mediate cell survival in DRGs².

Storage before reconstitution

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Sterile water, DMEM at a concentration of at least 10 µg/ml. Centrifuge all product preparations before use (10000 x g 5 min). Repeated freezing/thawing might result in loss of activity.

Storage after reconstitution

Up to one week at 4°C or four-six weeks at -70°C.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For more information, please refer to individual certificate of analysis for each product.

For research purposes only, not for human use

Shipping and Ordering Information

Free International Shipping

Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

For more country-specific shipping and contact information see Ordering & Shipping.

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Order Summary

Certificate of Analysis

Human proNGF Basic Research Pack