Overview
Type: Synthetic peptide
Form: Lyophilized powder
Human TNF Receptor I (extracellular) Blocking Peptide (#BLP-NT041) is the original antigen used for immunization during Anti-Human TNF Receptor I (extracellular) Antibody (#ANT-041) generation. The blocking peptide binds and ‘blocks’ Anti-Human TNF Receptor I (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human MCF-7 breast adenocarcinoma cell line lysate:1. Anti-Human TNF Receptor I (extracellular) Antibody (#ANT-041), (1:200).
2. Anti-Human TNF Receptor I (extracellular) Antibody, preincubated with Human TNF Receptor I (extracellular) Blocking Peptide (#BLP-NT041).
Western blot analysis of human PANC-1 pancreas ductal adenocarcinoma cell line lysate:1. Anti-Human TNF Receptor I (extracellular) Antibody (#ANT-041), (1:200).
2. Anti-Human TNF Receptor I (extracellular) Antibody, preincubated with Human TNF Receptor I (extracellular) Blocking Peptide (#BLP-NT041).
Properties
Sequence
- CRKNQYRHYWSEN, corresponding to amino acid residues 127-139 of human TNFR1 (Accession P19438).
Accession (Uniprot) Number P19438
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
