Cat #: BLP-NT041
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- CRKNQYRHYWSEN, corresponding to amino acid residues 127-139 of human TNFR1 (Accession P19438).
Accession (Uniprot) Number P19438
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of human MCF-7 breast adenocarcinoma cell line lysate:1. Anti-Human TNF Receptor I (extracellular) Antibody (#ANT-041), (1:200).
2. Anti-Human TNF Receptor I (extracellular) Antibody, preincubated with Human TNF Receptor I (extracellular) Blocking Peptide (#BLP-NT041). - Western blot analysis of human PANC-1 pancreas ductal adenocarcinoma cell line lysate:1. Anti-Human TNF Receptor I (extracellular) Antibody (#ANT-041), (1:200).
2. Anti-Human TNF Receptor I (extracellular) Antibody, preincubated with Human TNF Receptor I (extracellular) Blocking Peptide (#BLP-NT041).
Last update: 02/01/2024