Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- (C)DEDLQSKLEAFKTK, corresponding to amino acid residues 40-53 of rat IBA1/AIF1 (Accession P55009).
Accession (Uniprot) Number P55009
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat spleen (lanes 1 and 3), rat testis (lanes 2 and 4) and mouse spleen (lanes 5 and 6) lysates:1, 2, 5. Anti-IBA1/AIF1 Antibody (1:200), (#ACS-010).
3, 4, 6. Anti-IBA1/AIF1 Antibody, preincubated with IBA1/AIF1 Blocking Peptide (#BLP-CS010).- Rat brain sections.
Western blot analysis of rat spleen lysate (lanes 1 and 4), mouse spleen membranes (lanes 2 and 5) and mouse BV-2 microglia cell line lysate (lanes 3 and 6):1-3. Guinea Pig Anti-IBA1/AIF1 Antibody (#ACS-010-GP), (1:200).
4-6. Guinea Pig Anti-IBA1/AIF1 Antibody (#ACS-010-GP), preincubated with IBA1/AIF1 Blocking Peptide (#BLP-CS010).
Last update: 02/01/2024
