Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized Powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- Peptide (C)NKPTGYGSSIRR, corresponding to amino acid residues 74 - 85 of rat IGF1 (Accession P08025).
Accession (Uniprot) Number P08025
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis with Anti-IGF1 antibody:1, 4. Recombinant rat IGF-I protein (#I-200), 100 ng.
2, 5. Recombinant human IGF1, 100 ng.
3, 6. Recombinant human IGF2, 100 ng.Lanes 1-3: Anti-IGF1 Antibody (#ANT-046), (1:200).
Lanes 4-6: Anti-IGF1 Antibody, preincubated with IGF1 Blocking Peptide (BLP-NT046).Note that the antibody recognizes IGF1 (rat and human), but fails to recognize the closely related IGF2 protein.
Last update: 02/01/2024
