Cat #: BLP-NR208
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized Powder
Applications: wb
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Accession (Uniprot) Number Q8R366
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 2 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):1-2. Anti-IGSF8 (extracellular) Antibody (#ANR-208), (1:200).
3-4. Anti-IGSF8 (extracellular) Antibody, preincubated with IGSF8 (extracellular) Blocking Peptide (BLP-NR208). - Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 5), human Burkitt's lymphoma (Daudi) cell line lysate (lanes 2 and 6), human normal skin fibroblast Malme-3 cell line lysate (lanes 3 and 7) and Human prostate carcinoma LN-CaP cell line lysate (lanes 4 and 8):1-4. Anti-IGSF8 (extracellular) Antibody (#ANR-208), (1:200).
5-8. Anti-IGSF8 (extracellular) Antibody, preincubated with IGSF8 (extracellular) Blocking Peptide (BLP-NR208).
Last update: 15/12/2024