Overview
Type: Synthetic peptide
Form: Lyophilized powder
IP3 Receptor-1/ITPR1 Blocking Peptide (#BLP-CC019) is the original antigen used for immunization during Anti-IP3 Receptor-1 (ITPR1) Antibody (#ACC-019) generation. The blocking peptide binds and ‘blocks’ Anti-IP3 Receptor-1/ITPR1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-IP3 Receptor-1 (ITPR1) Antibody (#ACC-019), (1:2000).
2. Anti-IP3 Receptor-1 (ITPR1) Antibody, preincubated with IP3 Receptor-1/ITPR1 Blocking Peptide (#BLP-CC019).
Expression of IP3R1 in mouse cerebellumImmunohistochemical staining of mouse cerebellum with Anti-IP3 Receptor-1 (ITPR1) Antibody (#ACC-019). Immunoreactivity appears in Purkinje cells (arrows in A) and their dendritic trees. In addition, axonal processes coursing through cerebellar white matter are visualized (arrow in B).
Properties
Sequence
- (C)RIGLLGHPPHMNVNPQQPA, corresponding to amino acid residues 2732-2750 of rat IP3R1 (Accession P29994).
Accession (Uniprot) Number P29994
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
