Overview
Type: Synthetic peptide
Form: Lyophilized powder
IQGAP1 Blocking Peptide (#BLP-PZ055) is the original antigen used for immunization during Anti-IQGAP1 Antibody (#APZ-055) generation. The blocking peptide binds and ‘blocks’ Anti-IQGAP1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1, 2. Anti-IQGAP1 Antibody (#APZ-055), (1:200).
3, 4. Anti-IQGAP1 Antibody, preincubated with IQGAP1 Blocking Peptide (#BLP-PZ055) (#BLP-PZ055).
Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 3) and human MCF-7 breast adenocarcinoma cell line lysate (lanes 2 and 4):1, 2. Anti-IQGAP1 Antibody (#APZ-055), (1:200).
3, 4. Anti-IQGAP1 Antibody, preincubated with IQGAP1 Blocking Peptide (#BLP-PZ055) (#BLP-PZ055).
Expression of IQGAP1 in mouse cerebellumImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-IQGAP1 Antibody (#APZ-055), (1:200), followed by goat anti-rabbit-AlexaFluor-488. IQGAP1 immunoreactivity appears in Purkinje neurons (horizontal arrows) and their dendrites (vertical arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)KAYQDRLAYLRSHKDE, corresponding to amino acid residues 793 - 808 of human IQGAP1 (Accession P46940).
Accession (Uniprot) Number P46940
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Standard Quality Control Of Each Lot Western blot analysis.
