Cat #: AK-390_KIT
Size: 25 Vials
Form: Lyophilized
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Species reactivity key:
H- Human, M- Mouse, R- Rat
Anti-KCNN1 (KCa2.1, SK1) Antibody Cat #: APC-039
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, WB
Reactivity:
M, R
Immunogen
Peptide (C)DRPGSGKPPTVSHRLGHRR corresponding to amino acid residues 75-93 of rat KCNN1 (Accession P70606). Intracellular, N-terminal part.
Homology
Mouse - 17/19 amino acid residues identical; human - 12/19 amino acid residues identical.
KCNN1/KCa2.1 Blocking Peptide Cat #: BLP-PC039
Size:
40 µg
Anti-GIRK1 (Kir3.1) Antibody Cat #: APC-005
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
GST fusion protein with the sequence LQRISSVPGNSEEKLVSKTTKMLSDPMSQSVADLPPKLQKMAGGPTRMEGNLPAKLRKMNSDRFT, corresponding to residues 437-501 of mouse GIRK1 (Accession P63250). Intracellular, C-terminus.
Homology
Rat - identical; human - 64/66 amino acid residues identical; guinea pig - 63/66 amino acid residues identical; chicken - 59/66 amino acid residues identical.
GIRK1/Kir3.1 Blocking Peptide Cat #: BLP-PC005
Size:
0.12 mg
Mouse Anti-GIRK1 (Kir3.1) (extracellular) Antibody Cat #: ALM-031
Size:
25 µg
Host: Mouse
Type: Monoclonal
Application:
IC, IF, LCI, WB
Reactivity:
H, M
Immunogen
Synthetic peptide mapping to the extracellular loop of human GIRK1 (Accession P48549).
Anti-GIRK2 (Kir3.2) Antibody Cat #: APC-006
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
GST fusion protein with the sequence ELANRAEVPLSWSVS SKLNQHAELETEEEEKNPEELTERNG, corresponding to residues 374-414 of mouse Kir3.2 (Accession P48542). Intracellular, C-terminal part.
Homology
Rat - 40/41 amino acid residues identical; golden hamster - 39/41 amino acid residues identical; human - 37/41 amino acid residues identical.
GIRK2/Kir3.2 Blocking Peptide Cat #: BLP-PC006
Size:
0.12 mg
Anti-KCNK2 (TREK-1) Antibody Cat #: APC-047
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide DPKSAAQNSKPRLSFSTK(C), corresponding to residues 8-25 of human KCNK2 (Accession O95069). Intracellular, N-terminus.
Homology
Rat - identical; mouse - 17/18 amino acid residues identical.
KCNK2/TREK-1 Blocking Peptide Cat #: BLP-PC047
Size:
40 µg
Guinea pig Anti-KCNK2 (TREK-1) Antibody Cat #: APC-047-GP
Size:
50 µl
Host: Guinea pig
Type: Polyclonal
Application:
IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide DPKSAAQNSKPRLSFSTK(C), corresponding to residues 8-25 of human KCNK2 (Accession O95069). Intracellular, N-terminus.
Homology
Rat - identical; mouse - 17/18 amino acid residues identical.
KCNK2/TREK-1 Blocking Peptide Cat #: BLP-PC047
Size:
40 µg
Anti-KCNK3 (TASK-1) Antibody Cat #: APC-024
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, R
Immunogen
Peptide (C)EDEKRDAEHRALLTRNGQ, corresponding to amino acid residues 252-269 of human KCNK3 (Accession O14649). Intracellular, C-terminal part.
Homology
Rat, mouse - 17/18 amino acid residues identical. Homology with human TASK-3: 11/17 amino acid residues identical.
KCNK3/TASK-1 Blocking Peptide Cat #: BLP-PC024
Size:
40 µg
Anti-KV1.1 (KCNA1) Antibody Cat #: APC-009
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
GST fusion protein with the sequence HRETEGEEQAQLLHV SSPNLASDSDLSRRSSSTISKSEYMEIEEDMNNSIAHYRQANIRTGNCTTADQNCVNKSKLLTDV, corresponding to amino acid residues 416-495 of mouse KV1.1 (Accession P16388). Intracellular, C-terminus.
Homology
Rat - 78/80 amino acid residues identical; human - 76/80 amino acid residues identical; Xenopus Laevis - 70/80 amino acid residues identical.
Kv1.1/KCNA1 Blocking Peptide Cat #: BLP-PC009
Size:
0.12 mg
Anti-KV1.1 (KCNA1) (extracellular) Antibody Cat #: APC-161
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IFC, LCI, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)KDDKDFTGTIHRID, corresponding to amino acid residues 193-206 of rat KV1.1 (Accession P10499). 1st extracellular loop.
Homology
Mouse - identical; human - 13/14 amino acid residues identical.
Kv1.1/KCNA1 (extracellular) Blocking Peptide Cat #: BLP-PC161
Size:
40 µg
Anti-KV4.2 Antibody Cat #: APC-023
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)SNQLQSSEDEPAFVSK, corresponding to amino acid residues 454-469 of rat KV4.2 (Accession Q63881). Intracellular, C-terminus.
Homology
Mouse, human - 15/16 amino acid residues identical.
Kv4.2 Blocking Peptide Cat #: BLP-PC023
Size:
40 µg
Guinea pig Anti-KV4.2 Antibody Cat #: APC-023-GP
Size:
50 µl
Host: Guinea pig
Type: Polyclonal
Application:
IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)SNQLQSSEDEPAFVSK, corresponding to amino acid residues 454-469 of rat KV4.2 (Accession Q63881). Intracellular, C-terminus.
Homology
Mouse, human - 15/16 amino acid residues identical.
Kv4.2 Blocking Peptide Cat #: BLP-PC023
Size:
40 µg
Anti-KCNQ3 Antibody Cat #: APC-051
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
M, R
Immunogen
Peptide AEGEKKEDNRYSDLKTIIC, corresponding to amino acid residues 668-686 of rat KCNQ3 (Accession O88944). Intracellular, C-terminal.
Homology
Mouse - identical; human - 18/19 amino acid residues identical.
KCNQ3 Blocking Peptide Cat #: BLP-PC051
Size:
40 µg
Anti-KCNQ2 Antibody Cat #: APC-050
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)RGPTITDKDRTKGPAE, corresponding to amino acid residues 578-593 of rat KCNQ2 (Accession O88943). Intracellular, C-terminus.
Homology
Mouse - identical; human - 15/16 amino acid residues identical.
KCNQ2 Blocking Peptide Cat #: BLP-PC050
Size:
40 µg
Antibody Storage & Reconstitution Instructions
Storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Add deionized water, depending on the sample size.
Storage after reconstitution
The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control antigen storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution
100 μl water.
Control antigen storage after reconstitution
-20ºC.
Preadsorption control
1 μg peptide per 1 μg antibody.
Note
A mouse monoclonal antibody (#ALM-031) and guinea pig polyclonal antibodies (#APC-023-GP & #APC-047-GP) are included in this Explorer Kit. Please take into account when reacting with a secondary antibody.
For research purposes only, not for human use
