Overview
Lyophilized Powder yes
Origin Nocardiopsis sp. (soil fungi).
Source Natural
MW: 453
Purity: >98%
Effective concentration 50-500 nM.
Structure
Chemical name (9R,10S,12S)-2,3,9,10,11,12-Hexahydro-10-hydroxy-9-methyl-1-oxo-9,12-epoxy-1H-diindolo[1,2,3-fg:3',2',1'-kl]pyrrolo[3,4-i][1,6]benzodiazocine-10-carboxylic acid.
Molecular formula C26H19N3O5.
CAS No.: 99570-78-2
Activity K252b is a less potent derivative of K252a and inhibits a variety of kinases including PKC (IC50 = 20 nM), PKA (IC50 = 90 nM) and PKG (IC50 = 100 nM)1. It also inhibits MLCK (IC50 = 147 nM) and CaMK (IC50 = 12 nM).
References-Activity
- Kase, H. et al. (1987) Biochem. Biopphys. Res. Commun. 142, 436.
Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Solubility DMSO or DMF. Centrifuge all product preparations before use (10000 x g 5 min).
Storage of solutions Up to one week at 4°C or three months at -20°C.
Protect from light.
Protect from light.
Our bioassay
Alomone Labs K252b inhibits MARCKS phosphorylation via PKC in C6 glioma cells.Cells were grown to 70% confluence, and then serum starved for 18 h. The cells were preincubated for 30 min with different concentrations of K252b (#K-170) and stimulated with 2 µM PMA (#P-800) for 30 min. Cell proteins were resolved by SDS-PAGE and probed with anti-phospho (ser)- MARCKS (myristoylated alanine-rich protein kinase C substrate).
Alomone Labs K252b inhibits tyrosine phosphorylation of TrKA in PC12 cells.Cells were incubated in the presence or absence of 10 ng/ml NGF with or without various concentrations of K252b (#K-170). The inhibitory effect of K252b on neurite outgrowth stimulated by NGF is presented.
References - Scientific background
- Yasuzawa T. et al. (1986) J. Antibiotics 39, 1072.
- Ross, A.H. et al. (1995) J. Neurochem. 65, 2748.
- Kojima, M. et al. (1995) J. Neurosci Res. 42, 775.
- Galter, D. and Unsicker, K. (2000) J. Neurosci Res. 61, 295.
- Taoka, M. et al. (1990) Biochem Biophys Res Commun. 170, 1151.
Scientific background
K252b is an alkaloid isolated from Nocardiopsis sp. soil fungi. It is a less potent derivative of K252a,3-5 cell-permeable protein kinase inhibitor.
This compound potentiates neurotrophin-3 activity in certain neurons by an unknown mechanism. In other neurons it serves as a control to K252a since it accumulates in the plasma membrane of the neuron.1
Lyophilized Powder
K252b (#K-170) is a highly pure, natural, and biologically active compound.
For research purposes only, not for human use
Last Update: 02/01/2024
Applications
Citations
Citations
Powered by Bioz