Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCC1/SLC12A4 Blocking Peptide (#BLP-KT001) is the original antigen used for immunization during Anti-KCC1 (SLC12A4) Antibody (#AKT-001) generation. The blocking peptide binds and ‘blocks’ Anti-KCC1/SLC12A4 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human K562 erythroleukemia cell lysates (lanes 3 and 6):1-3. Anti-KCC1 (SLC12A4) Antibody (#AKT-001), (1:200).
4-6. Anti-KCC1 (SLC12A4) Antibody, preincubated with KCC1/SLC12A4 Blocking Peptide (#BLP-KT001).
Properties
Sequence
- (C)HAPDNFRELVHIK, corresponding to amino acid residues 998-1010 of rat KCC1 (Accession Q63632).
Accession (Uniprot) Number Q63632
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
