Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- (C)KNPANTRLRLNVPE, corresponding to amino acid residues 967-980 of rat KCC2 (Accession Q63633).
Accession (Uniprot) Number Q63633
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-KCC2 (SLC12A5) Antibody (#AKT-005), (1:500).
2. Anti-KCC2 (SLC12A5) Antibody, preincubated with KCC2/SLC12A5 Blocking Peptide (#BLP-KT005).
Western blot analysis of mouse brain lysates:1. Anti-KCC2 (SLC12A5) Antibody (#AKT-005), (1:200).
2. Anti-KCC2 (SLC12A5) Antibody, preincubated with KCC2/SLC12A5 Blocking Peptide (#BLP-KT005).- Rat brain sections (1:1000).
Last update: 02/01/2024
