Overview
Type: Synthetic peptide
Form: Lyophilized powder
KChIP2 Blocking Peptide (#BLP-PC142) is the original antigen used for immunization during Anti-KChIP2 Antibody (#APC-142) generation. The blocking peptide binds and ‘blocks’ Anti-KChIP2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain lysate:1. Anti-KChIP2 Antibody (#APC-142), (1:200).
2. Anti-KChIP2 Antibody, preincubated with KChIP2 Blocking Peptide (#BLP-PC142).
Western blot analysis of rat heart (lanes 1 and 3) and rat hippocampus (lanes 2 and 4) lysate:1,2. Anti-KChIP2 Antibody (#APC-142), (1:200).
3,4. Anti-KChIP2 Antibody, preincubated with KChIP2 Blocking Peptide (#BLP-PC142).
Expression of KChIP2 in mouse hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-KChIP2 Antibody (#APC-142), (1:400), followed by goat-anti-rabbit-Alexa-488 (green). KChIP2 staining in the hippocampal CA1 region appears in neuronal profiles (arrows) in the pyramidal layer (Pyr). DAPI is used to stain nuclei (blue).
Properties
Sequence
- (C)RGQGRKESLSDSRD, corresponding to amino acid residues 2-15 of human KChIP2 (Accession Q9NS61).
Accession (Uniprot) Number Q9NS61
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
