Overview
Type: Synthetic peptide
Form: Lyophilized powder
KChIP3/CSEN Blocking Peptide (#BLP-PC143) is the original antigen used for immunization during Anti-KChIP3 (CSEN, DREAM) Antibody (#APC-143) generation. The blocking peptide binds and ‘blocks’ Anti-KChIP3/CSEN primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human T-cell lymphoma Jurkat cell lysates (lanes 1 and 4), mouse heart lysates (lanes 2 and 5) and rat brain lysates (lanes 3 and 6):1-3. Anti-KChIP3 (CSEN, DREAM) Antibody (#APC-143), (1:200).
4-6. Anti-KChIP3 (CSEN, DREAM) Antibody, preincubated with KChIP3/CSEN Blocking Peptide (#BLP-PC143).
Properties
Sequence
- (C)SKREGIKWQRPR, corresponding to amino acid residues 18-31 of rat KChIP3 (Accession Q9JM47).
Accession (Uniprot) Number Q9JM47
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
