Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNC3/Kv3.3 Blocking Peptide (#BLP-PC102) is the original antigen used for immunization during Anti-KCNC3 (KV3.3) Antibody (#APC-102) generation. The blocking peptide binds and ‘blocks’ Anti-KCNC3/Kv3.3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blotting of rat brain membranes:1. Anti-KCNC3 (KV3.3) Antibody (#APC-102), (1:200).
2. Anti-KCNC3 (KV3.3) Antibody, preincubated with KCNC3/Kv3.3 Blocking Peptide (#BLP-PC102).- Rat brain sections.
Properties
Sequence
- KSPITPGSRGRYSRDRAC, corresponding to amino acid residues 701-718 of rat KCNC3 (Accession Q01956).
Accession (Uniprot) Number Q01956
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
