Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNC4/Kv3.4 Blocking Peptide (#BLP-PC019) is the original antigen used for immunization during Anti-KCNC4 (KV3.4) Antibody (#APC-019) generation. The blocking peptide binds and ‘blocks’ Anti-KCNC4/Kv3.4 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysates:1. Anti-KCNC4 (KV3.4) Antibody (#APC-019), (1:200).
2. Anti-KCNC4 (KV3.4) Antibody, preincubated with KCNC4/Kv3.4 Blocking Peptide (#BLP-PC019).- Rat brain sections.
Mouse transversus abdominus (1:100-1:1000) (Brooke, R.E. et al. (2004) Eur. J. Neurosci. 20, 3313.).
Properties
Sequence
- EAGDD ERELA LQRLG PHEG(C), corresponding to amino acid residues 177-195 of rat KCNC4 (Accession Q63734).
Accession (Uniprot) Number Q63734
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
