Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNE1/IsK Blocking Peptide (#BLP-PC163) is the original antigen used for immunization during Anti-KCNE1 (IsK) Antibody (#APC-163) generation. The blocking peptide binds and ‘blocks’ Anti-KCNE1/IsK primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat kidney (lanes 1 and 4), rat heart (lanes 2 and 5) and mouse kidney (lanes 3 and 6) membranes:1-3. Anti-KCNE1 (IsK) Antibody (#APC-163), (1:200).
4-6. Anti-KCNE1 (IsK) Antibody, preincubated with KCNE1/IsK Blocking Peptide (#BLP-PC163).
Properties
Sequence
- (C)RSKKLEHSHDPFN, corresponding to amino acid residues 68-80 of rat KCNE1 (Accession P15383).
Accession (Uniprot) Number P15383
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
