Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNE2/MiRP1 Blocking Peptide (#BLP-PC054) is the original antigen used for immunization during Anti-KCNE2 (MiRP1) Antibody (#APC-054) generation. The blocking peptide binds and ‘blocks’ Anti-KCNE2/MiRP1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat heart membranes:1. Anti-KCNE2 (MiRP1) Antibody (#APC-054), (1:200).
2. Anti-KCNE2 (MiRP1) Antibody, preincubated with KCNE2/MiRP1 Blocking Peptide (#BLP-PC054).
Expression of KCNE2 in mouse heartImmunohistochemical staining of mouse heart frozen sections using Anti-KCNE2 (MiRP1) Antibody (#APC-054), (red). Immunoreactivity appears as a striated pattern in heart muscle.
Properties
Sequence
- (C)IVEDWQQKYRSQILHLEDSK, corresponding to amino acid residues 88-107 of rat KCNE2 (Accession P63161).
Accession (Uniprot) Number P63161
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
