Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNF1/Kv5.1 Blocking Peptide (#BLP-PC134) is the original antigen used for immunization during Anti-KCNF1 (KV5.1) Antibody (#APC-134) generation. The blocking peptide binds and ‘blocks’ Anti-KCNF1/Kv5.1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain lysate (lanes 1 and 4), human SHSY-5Y neuroblastoma cell line lysate (lanes 2 and 5) and rat brain membranes (lanes 3 and 6):1-3. Anti-KCNF1 (KV5.1) Antibody (#APC-134), (1:400).
4-6. Anti-KCNF1 (KV5.1) Antibody, preincubated with KCNF1/Kv5.1 Blocking Peptide (#BLP-PC134).
Properties
Sequence
- (C)K(S)VWKFLEKPESS, corresponding to amino acid residues 166-178 of mouse KV5.1 (Accession Q7TSH7).
Accession (Uniprot) Number Q7TSH7
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 2 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
